Journal:

Article Title: Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization

doi: 10.1128/JCM.39.7.2627-2633.2001

Figure Lengend Snippet: Specificity test of oligonucleotide probe pmhyb449 and its complementary non-pmhyb449 probe labeled by Cy3 with bacteria grown in pure culture

Article Snippet: Twenty-two strains of P. multocida and other bacterial species isolated from normal and diseased lungs of poultry and other animals were obtained from publicly accessible service collections and a local culture collection (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Bacterial species Animal source Hybridization signal intensity with b : pmhyb449 Non-pmhyb449 NCTC 10322 T P. multocida subsp. multocida Pig +++ − 214 P. multocida subsp. multocida Calf +++ − 40605-1 P. multocida subsp. multocida Eider +++ − RA 12/2 P. multocida subsp. multocida Calf +++ − NCTC 10204 T P. multocida subsp. gallicida Cow +++ − 77179 P. multocida subsp. gallicida Chicken +++ − NCTC 11619 T P. multocida subsp. septica Human c +++ − 5 P. avium biovar 2 Calf +++ − 25 P. canis biovar 2 Calf +++ − NCTC 11188 T P. gallinarum Chicken − − ATCC 43326 T P. canis biovar 1 Dog − − F 149 T P. anatis Duck − − CCM 5974 T Actinobacillus salpingitidis Chicken − − NCTC 4189 T A. lignieresii Cow − − P737 Mannheimia glucosida Sheep − − 14R525 E. coli Human − − SA 4461 Haemophilus paragallinarum Chicken − − SA 7191 H. paragallinarum Chicken − − 4237/2sv Riemerella anatipestifer Duck − − 4280/2sv R. anatipestifer Duck − − 726-82 T Coenonia anatina Duck − − 1912+pSD S. enterica serotype Gallinarum Chicken − − 19110+pSD S. enterica serotype Gallinarum Chicken − − Open in a separate window a A superscript T indicates the type strain. b +++, high signal intensity; −, no signal observed. c Infection was by means of a cat bite.

Techniques: Labeling, Hybridization

Journal:

Article Title: Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization

doi: 10.1128/JCM.39.7.2627-2633.2001

Figure Lengend Snippet: Oligonucleotide probe DNA sequence of the P. multocida -specific probe pmhyb449 compared with selected 16S rRNA sequences of strains of bacteria within the genera Pasteurella, Actinobacillus , and Mannheimia used for ISH

Article Snippet: Twenty-two strains of P. multocida and other bacterial species isolated from normal and diseased lungs of poultry and other animals were obtained from publicly accessible service collections and a local culture collection (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Bacterial species Animal source Hybridization signal intensity with b : pmhyb449 Non-pmhyb449 NCTC 10322 T P. multocida subsp. multocida Pig +++ − 214 P. multocida subsp. multocida Calf +++ − 40605-1 P. multocida subsp. multocida Eider +++ − RA 12/2 P. multocida subsp. multocida Calf +++ − NCTC 10204 T P. multocida subsp. gallicida Cow +++ − 77179 P. multocida subsp. gallicida Chicken +++ − NCTC 11619 T P. multocida subsp. septica Human c +++ − 5 P. avium biovar 2 Calf +++ − 25 P. canis biovar 2 Calf +++ − NCTC 11188 T P. gallinarum Chicken − − ATCC 43326 T P. canis biovar 1 Dog − − F 149 T P. anatis Duck − − CCM 5974 T Actinobacillus salpingitidis Chicken − − NCTC 4189 T A. lignieresii Cow − − P737 Mannheimia glucosida Sheep − − 14R525 E. coli Human − − SA 4461 Haemophilus paragallinarum Chicken − − SA 7191 H. paragallinarum Chicken − − 4237/2sv Riemerella anatipestifer Duck − − 4280/2sv R. anatipestifer Duck − − 726-82 T Coenonia anatina Duck − − 1912+pSD S. enterica serotype Gallinarum Chicken − − 19110+pSD S. enterica serotype Gallinarum Chicken − − Open in a separate window a A superscript T indicates the type strain. b +++, high signal intensity; −, no signal observed. c Infection was by means of a cat bite.

Techniques: Sequencing

Journal:

Article Title: Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization

doi: 10.1128/JCM.39.7.2627-2633.2001

Figure Lengend Snippet: Photomicrographs demonstrating specific detection of P. multocida in pure culture and formalin-fixed lung tissues by use of the FISH technique. Shown are a pure culture of P. multocida subsp. multocida strain 40605-1 (A) and lung tissue sections of chicken (B, C, D, E, and F) or pig (G, H, and I) following light microscopy (C) or epifluorescence microscopy (A, B, and D through I) and ISH with the P. multocida-specific Cy3-labeled probe pmhyb449 (A, B, D, E, G, and H) and a nonsense Cy3-labeled probe, non-pmhyb449 (F and I), used as a control. A normal chicken lung (C) shows the location of the bacteria detected in cases of fowl cholera with an indication of the parabronchi (1), infundibulum (2), air capillary (3), pleura (4), and arterioles (5) (hematoxylin and eosin stain). Also shown are chicken lung infected with fowl cholera (experimentally infected with P. multocida subsp. multocida strain 40605-1), with the location of P. multocida cells near two blood vessels (artery and arteriole) (B), with P. multocida cells within the lumen and the walls of the air capillaries and perivascular space (D), and with P. multocida cells as individual organisms or as microcolonies (E); a control tissue section hybridized with probe non-pmhyb449 is also shown (F). Also shown is lung of a pig infected postmortem with P. multocida subsp. septica strain HIM 746-6T showing P. multocida bacteria in the lumen and wall of the alveoli (G), the area close to the pleura (H), and a control tissue section hybridized with non-pmhyb449 (I). Bars = 10 μm (A, B, D, E, F, G, H, and I) and 100 μm (C).

Article Snippet: Twenty-two strains of P. multocida and other bacterial species isolated from normal and diseased lungs of poultry and other animals were obtained from publicly accessible service collections and a local culture collection (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Bacterial species Animal source Hybridization signal intensity with b : pmhyb449 Non-pmhyb449 NCTC 10322 T P. multocida subsp. multocida Pig +++ − 214 P. multocida subsp. multocida Calf +++ − 40605-1 P. multocida subsp. multocida Eider +++ − RA 12/2 P. multocida subsp. multocida Calf +++ − NCTC 10204 T P. multocida subsp. gallicida Cow +++ − 77179 P. multocida subsp. gallicida Chicken +++ − NCTC 11619 T P. multocida subsp. septica Human c +++ − 5 P. avium biovar 2 Calf +++ − 25 P. canis biovar 2 Calf +++ − NCTC 11188 T P. gallinarum Chicken − − ATCC 43326 T P. canis biovar 1 Dog − − F 149 T P. anatis Duck − − CCM 5974 T Actinobacillus salpingitidis Chicken − − NCTC 4189 T A. lignieresii Cow − − P737 Mannheimia glucosida Sheep − − 14R525 E. coli Human − − SA 4461 Haemophilus paragallinarum Chicken − − SA 7191 H. paragallinarum Chicken − − 4237/2sv Riemerella anatipestifer Duck − − 4280/2sv R. anatipestifer Duck − − 726-82 T Coenonia anatina Duck − − 1912+pSD S. enterica serotype Gallinarum Chicken − − 19110+pSD S. enterica serotype Gallinarum Chicken − − Open in a separate window a A superscript T indicates the type strain. b +++, high signal intensity; −, no signal observed. c Infection was by means of a cat bite.

Techniques: Light Microscopy, Epifluorescence Microscopy, Labeling, H&E Stain, Infection